The inability of a bacteriophage to infect <i>Staphylococcus aureus</i> does not prevent it from specifically delivering a photosensitizer to the bacterium enabling its lethal photosensitization



Hope, CK ORCID: 0000-0003-2986-0824, Packer, S, Wilson, M and Nair, SP
(2009) The inability of a bacteriophage to infect <i>Staphylococcus aureus</i> does not prevent it from specifically delivering a photosensitizer to the bacterium enabling its lethal photosensitization. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 64 (1). pp. 59-61.

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Abstract

<h4>Objectives</h4>It has been demonstrated that the efficiency of lethal photosensitization can be improved by covalently binding photosensitizing agents to bacteriophage. In this study we have investigated whether a bacteriophage requires the capacity to infect the bacterium to enhance lethal photosensitization when linked to a photosensitizer.<h4>Methods</h4>Tin (IV) chlorin e6 (SnCe6) was conjugated to bacteriophage Phi11, a transducing phage that can infect Staphylococcus aureus NCTC 8325-4, but not epidemic methicillin-resistant S. aureus (EMRSA)-16. The conjugate and appropriate controls were incubated with these bacteria and either exposed to laser light at 632.8 nm or kept in the dark.<h4>Results</h4>The SnCe6/Phi11 conjugate achieved a statistically significant reduction in the number of viable bacteria of both 8325-4 and EMRSA-16 strains by 2.31 log(10) and 2.63 log(10), respectively. The conjugate could not however instigate lethal photosensitization of Escherichia coli. None of the other combinations of controls, such as an equivalent concentration of SnCe6 only, an equivalent titre of bacteriophage only or experiments conducted without laser light, yielded significant reductions in the number of viable bacteria recovered.<h4>Conclusions</h4>The inability of a bacteriophage to infect S. aureus does not prevent it from specifically delivering a photosensitizer to a bacterium enabling its lethal photosensitization.

Item Type: Article
Additional Information: ## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords: tin (IV) chlorin e6, SnCe6, MRSA, photodynamic therapy, PDT
Subjects: ?? RK ??
Divisions: Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry
Depositing User: Symplectic Admin
Date Deposited: 16 Oct 2009 09:25
Last Modified: 13 Oct 2023 09:08
DOI: 10.1093/jac/dkp157
Publisher's Statement : This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The definitive publisher-authenticated version 'C. K. Hope , S. Packer , M. Wilson , and S. P. Nair The inability of a bacteriophage to infect Staphylococcus aureus does not prevent it from specifically delivering a photosensitizer to the bacterium enabling its lethal photosensitization JAC Advance Access published on July 1, 2009, DOI 10.1093/jac/dkp157. J. Antimicrob. Chemother. 64: 59-61.' is available online at: http://dx.doi.org/doi:10.1093/jac/dkp157
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URI: https://livrepository.liverpool.ac.uk/id/eprint/1169