Sequential action of R- and K-specific gingipains of Porphyromonas gingivalis in the generation of the haem-containing pigment from oxyhaemoglobin.



Smalley, John W, Birss, Andrew J, Szmigielski, Borys and Potempa, Jan
(2007) Sequential action of R- and K-specific gingipains of Porphyromonas gingivalis in the generation of the haem-containing pigment from oxyhaemoglobin. Archives of biochemistry and biophysics, 465 (1). pp. 44-49.

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Abstract

The arginine- and lysine-specific gingipains of Porphyromonas gingivalis have been implicated in the degradation of haemoglobin from which the black mu-oxo haem dimer-containing pigment is generated. Here, we examined interactions of oxyhaemoglobin (oxyHb) with the Arg-(R)-specific (HRgpA) and Lys-(K)-specific (Kgp) gingipains. Incubation of oxyHb with HRgpA resulted in formation of methaemoglobin (metHb), which could be prevented by the R-gingipain specific inhibitor leupeptin. oxyHb-Kgp interactions resulted in formation of a haemoglobin haemichrome. This was inhibited by the lysine-specific protease inhibitor Z-Phe-Lys-acyloxymethylketone (Z-FKck). metHb, formed by treatment of oxyHb with either NaNO(2) or by pre-incubation with HRgpA, was rapidly degraded by Kgp compared to oxyHb. metHb degradation by Kgp was also inhibited Z-FKck. Together these data show that R-gingipain activity is crucial for converting oxyHb into the metHb form which is rendered more susceptible to Kgp degradation for the eventual release of iron(III) protoporphyrin IX and production of the mu-oxo haem dimer. This explains previous observations [J.W. Smalley, M.F. Thomas, A.J. Birss, R. Withnall, J. Silver, Biochem. J. 379 (2004) 833-840.] of the requirement for a combination of both R- and K-gingipains for pigment production from oxyhaemoglobin by P. gingivalis.

Item Type: Article
Additional Information: Journal Article United States## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords: Porphyromonas gingivalis, Heme, Cysteine Endopeptidases, Adhesins, Bacterial, Oxyhemoglobins, Hemeproteins, Plant Extracts, Kinetics, Pigments, Biological, Gingipain Cysteine Endopeptidases
Subjects: ?? RK ??
Divisions: Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry
Depositing User: Symplectic Admin
Date Deposited: 17 Feb 2010 13:09
Last Modified: 15 Mar 2024 06:53
DOI: 10.1016/j.abb.2007.05.011
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/1227