The role and origin of uterine natural killer cells in patients with unexplained recurrent miscarriage


Karam, Asiyah The role and origin of uterine natural killer cells in patients with unexplained recurrent miscarriage. Master of Philosophy thesis, University of Liverpool.

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Abstract

The aim of the study was to determine the role of uNK cells by looking at their numbers across three location sites, the epithelial edge, areas of low stromal cell density/oedema and peri-vascular stroma/spiral arteries. The universal NK cell marker, CD56 was used to identify uNK cells in the endometrium. The research determined whether uNK cells were the result of proliferation and differentiation of resident uNK cells or trafficking of pbNK cells. In addition, it was determined whether uNK cells play a part in spiral artery remodelling and trophoblast invasion and also the role they may play in RM. The hypothesis was tested using Immunohistochemical staining on serial sequential sections of endometrial tissue received from RM patients of extreme phenotype between LH + 5-9 days. Analysis was made on 20 RM patients, 10 patients were grouped as high uNK, defined as an uNK cell density value of ≥ 5% and 10 were grouped as low uNK, defined as an uNK cell density value of ≤ 2.5%. The endometrial samples were stained for the antibodies to CD56, KI67, NKp30, L-Selectin and CD16, uNK cell markers of proliferation, differentiation and trafficking. No association was found between RM and age. The high uNK density group had significantly higher levels for all the antibodies stained (P

Item Type: Thesis (Master of Philosophy)
Additional Information: Date: 2009-08 (completed)
Subjects: ?? RG ??
Divisions: Faculty of Health and Life Sciences
Depositing User: Symplectic Admin
Date Deposited: 27 Jan 2012 11:04
Last Modified: 16 Dec 2022 04:33
DOI: 10.17638/00001280
Supervisors:
URI: https://livrepository.liverpool.ac.uk/id/eprint/1280
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