PRKC-ζ Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention.



Yao, Sheng, Bee, Alix, Brewer, Daniel ORCID: 0000-0003-4753-9794, Dodson, Andrew, Beesley, Carol, Ke, Youqiang ORCID: 0000-0001-5341-8644, Ambroisine, Laurence, Fisher, Gabrielle, Møller, Heinrich ORCID: 0000-0001-8200-5929, Dickinson, Tim
et al (show 13 more authors) (2010) PRKC-ζ Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention. Genes & cancer, 1 (5). 444 - 464.

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Abstract

We show protein kinase C-zeta (PKC-ζ) to be a novel predictive biomarker for survival from prostate cancer (P < 0.001). We also confirm that transcription of the PRKC-ζ gene is crucial to the malignant phenotype of human prostate cancer. Following siRNA silencing of PRKC-ζ in PC3-M prostate cancer cells, stable transfectant cell line si-PRKC-ζ-PC3-M(T1-6) is phenotypically nonmalignant in vitro and in vivo. Genome-wide expression analysis identified 373 genes to be differentially expressed in the knockdown cells and 4 key gene networks to be significantly perturbed during phenotype modulation. Functional interconnection between some of the modulated genes is revealed, although these may be within different regulatory pathways, emphasizing the complexity of their mutual interdependence. Genes with altered expression following PRKC-ζ knockdown include HSPB1, RAD51, and ID1 that we have previously described to be critical in prostatic malignancy. Because expression of PRKC-ζ is functionally involved in promoting the malignant phenotype, we propose PKC-ζ as a novel and biologically relevant target for therapeutic intervention in prostate cancer.

Item Type: Article
Subjects: R Medicine > RK Dentistry
Divisions: ?? sch_dental ??
Depositing User: Symplectic Admin
Date Deposited: 02 Sep 2010 09:23
Last Modified: 22 Jan 2021 08:10
DOI: 10.1177/1947601910376079
Publisher's Statement : © The Author(s) 2010. Yao, Sheng, Bee, Alix, Brewer, Daniel, Dodson, Andrew, Beesley, Carol; Ke, Youqiang; Ambroisine, Laurence, Fisher, Gabrielle, Møller, Heinrich; Dickinson, Tim, Gerard, Patricia, Lian, Lu-Yu, Risk, Janet, Lane, Brian, Smith, Paul, Reuter, Victor, Berney, Daniel, Gosden, Christine, Scardino, Peter, Cuzick, Jack, Djamgoz, Mustafa B. A., Cooper, Colin and Foster, Christopher S. PRKC-ζ Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention Genes & Cancer May 2010 1: 444-464, first published 17 June 2010 http://dx.doi.org/10.1177/1947601910376079
URI: https://livrepository.liverpool.ac.uk/id/eprint/1357