Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing



Wilson, Robert, Bourne, Christian, Chaudhuri, Roy R, Gregory, Richard, Kenny, John and Cossins, Andrew ORCID: 0000-0002-0813-5212
(2014) Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing. PLOS ONE, 9 (6). e100572-.

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Abstract

The identification of nucleic acid aptamers would be advanced if they could be obtained after fewer rounds of selection and amplification. In this paper the identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods. This is significant because it shows that next generation sequencing and motif finding informatics have the potential to simplify the selection of aptamers by avoiding multiple rounds of enzymatic transcription and amplification.

Item Type: Article
Uncontrolled Keywords: Humans, Thrombin, Sequence Analysis, DNA, Computational Biology, Catalytic Domain, Base Sequence, Models, Molecular, SELEX Aptamer Technique, Aptamers, Nucleotide, High-Throughput Nucleotide Sequencing, Nucleotide Motifs
Divisions: Faculty of Health and Life Sciences
Depositing User: Symplectic Admin
Date Deposited: 26 Aug 2014 10:24
Last Modified: 16 Dec 2022 12:28
DOI: 10.1371/journal.pone.0100572
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/14753