Wilson, Robert, Bourne, Christian, Chaudhuri, Roy R, Gregory, Richard, Kenny, John and Cossins, Andrew ORCID: 0000-0002-0813-5212
(2014)
Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing.
PLOS ONE, 9 (6).
e100572-.
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Abstract
The identification of nucleic acid aptamers would be advanced if they could be obtained after fewer rounds of selection and amplification. In this paper the identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods. This is significant because it shows that next generation sequencing and motif finding informatics have the potential to simplify the selection of aptamers by avoiding multiple rounds of enzymatic transcription and amplification.
Item Type: | Article |
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Uncontrolled Keywords: | Humans, Thrombin, Sequence Analysis, DNA, Computational Biology, Catalytic Domain, Base Sequence, Models, Molecular, SELEX Aptamer Technique, Aptamers, Nucleotide, High-Throughput Nucleotide Sequencing, Nucleotide Motifs |
Divisions: | Faculty of Health and Life Sciences |
Depositing User: | Symplectic Admin |
Date Deposited: | 26 Aug 2014 10:24 |
Last Modified: | 16 Dec 2022 12:28 |
DOI: | 10.1371/journal.pone.0100572 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/14753 |