Effects of zinc and fluoride on the remineralisation of artificial carious lesions under simulated plaque fluid conditions.



Lynch, RJM, Churchley, D, Butler, A, Kearns, S, Thomas, GV, Badrock, TC, Cooper, L and Higham, SM ORCID: 0000-0002-4097-4702
(2011) Effects of zinc and fluoride on the remineralisation of artificial carious lesions under simulated plaque fluid conditions. Caries research, 45 (3). 313 - 322.

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Abstract

The aim was to study the effects of zinc (Zn) and fluoride (F) on remineralisation at plaque fluid concentrations. Artificial carious lesions were created in 2 acid-gel demineralising systems (initially infinitely undersaturated and partially saturated with respect to enamel) giving lesions with different mineral distribution characteristics (high and low R values, respectively) but similar integrated mineral loss values. Lesions of both types were assigned to 1 of 4 groups and remineralised for 5 days at 37°C. Zn and F were added, based on plaque fluid concentrations 1 h after application, to give 4 treatments: 231 μmol/l Zn, 10.5 μmol/l F, Zn/F combined and an unmodified control solution (non-F/non-Zn). Subsequently remineralisation was measured using microradiography. High-R lesions were analysed for calcium, phosphorus, F and Zn using electron probe micro-analysis. All lesions underwent statistically significant remineralisation. For low-R lesions, remineralisation was in the order F(a) < non-F/non-Zn(a) < Zn(a, b) < Zn/F(b), and for high-R lesions F(a) < non-F/non-Zn(b) < Zn(b) < Zn/F(c) (treatments with the same superscript letter not significantly different, at p < 0.05). Qualitatively, remineralisation occurred throughout non-F/non-Zn and Zn groups, predominantly at the surface zone (F) and within the lesion body (Zn/F). Electron probe micro-analysis revealed Zn in relatively large amounts in the outer regions (Zn, Zn/F). F was abundant not only at the surface (F), but also in the lesion body (Zn/F). Calcium:phosphate ratios were similar to hydroxyapatite (all). To conclude, under static remineralising conditions simulating plaque fluid, Zn/F treatment gave significantly greater remineralisation than did F treatment, possibly because Zn in the Zn/F group maintained greater surface zone porosity compared with F, facilitating greater lesion body remineralisation.

Item Type: Article
Uncontrolled Keywords: Dental Plaque, Dental Enamel, Animals, Cattle, Dental Caries, Durapatite, Fluorides, Calcium, Zinc, Phosphorus, Lactic Acid, Methylcellulose, Cariostatic Agents, Electron Probe Microanalysis, Microradiography, Spectrometry, X-Ray Emission, Tooth Remineralization, Temperature, Hydrogen-Ion Concentration, Time Factors
Subjects: R Medicine > RK Dentistry
Divisions: ?? sch_dental ??
Depositing User: Symplectic Admin
Date Deposited: 04 Aug 2011 09:46
Last Modified: 16 Sep 2020 08:45
DOI: 10.1159/000324804
Publisher's Statement : © 2011 S. Karger AG, Basel
URI: http://livrepository.liverpool.ac.uk/id/eprint/1481