Evaluation of Flinders Technology Associates cards for storage and molecular detection of avian metapneumoviruses

Awad, Faez, Baylis, Matthew ORCID: 0000-0003-0335-187X, Jones, Richard and Ganapathy, Kannan ORCID: 0000-0002-9401-3560 (2014) Evaluation of Flinders Technology Associates cards for storage and molecular detection of avian metapneumoviruses. Avian Pathology, 43 (02). pp. 125-129.

Text Final Version aMPV FTA card 04_06_12 ( Avian Pathology Journal .doc - Unspecified Download (210kB)

Abstract

The feasibility of using Flinders Technology Associates (FTA) cards for the molecular detection of avian metapneumovirus (aMPV) by reverse transcriptase-polymerase chain reaction (RT-PCR) was investigated. Findings showed that no virus isolation was possible from aMPV-inoculated FTA cards, confirming viral inactivation upon contact with the cards. The detection limits of aMPV from the FTA card and tracheal organ culture medium were 101.5 median ciliostatic doses/ml and 100.75 median ciliostatic doses/ml respectively. It was possible to perform molecular characterization of both subtypes A and B aMPV using inoculated FTA cards stored for up to 60 days at 4 to 6°C. Tissues of the turbinate, trachea and lung of aMPV-infected chicks sampled either by direct impression smears or by inoculation of the tissue homogenate supernatants onto the FTA cards were positive by RT-PCR. However, the latter yielded more detections. FTA cards are suitable for collecting and transporting aMPV-positive samples, providing a reliable and hazard-free source of RNA for molecular characterization.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	Animals, Chickens, Metapneumovirus, Paramyxoviridae Infections, RNA, Viral, Specimen Handling, Virus Inactivation, Sensitivity and Specificity, Reverse Transcriptase Polymerase Chain Reaction, Micropore Filters
Depositing User:	Symplectic Admin
Date Deposited:	08 Jan 2015 10:21
Last Modified:	15 Dec 2022 11:47
DOI:	10.1080/03079457.2014.885114
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2004403