The role of heparan sulphate in the development of mouse embryoid bodies

Hazeldine, Poppy
The role of heparan sulphate in the development of mouse embryoid bodies. Master of Philosophy thesis, University of Liverpool.

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The development of in vitro Embryoid Body (EB) formation facilitates the investigation of many aspects of cell differentiation occurring during early mammalian development. The function of various factors, such as fibroblast growth factors (FGFs) and their corresponding pathways within embryogenesis, has been widely studied. It is well documented that the polysaccharide Heparan Sulphate (HS) is essential for normal development within mouse embryonic stem cells (mESC); this is thought to be due its role as a co-factor in FGF signalling. Despite acting as a successful model for periimplantation, HS-deficient EBs have yet to be fully characterised and it is undetermined if they develop in a similar way to EBs generated from wild-type (WT) cells. The aim of this study was to investigate the role of HS in the development of mouse EBs at Day 4 derived from WT and EXT1-/- mESCs. The differentiation of Primitive Endoderm (PrE) and the subsequent synthesis of a basement membrane were primarily investigated using immunofluorescence and quantitative Polymerase Chain Reaction (qPCR). The expression of mesoderm, ectoderm and pluripotency markers were also analysed using these techniques.

Item Type: Thesis (Master of Philosophy)
Additional Information: Date: 2014-09 (completed)
Depositing User: Symplectic Admin
Date Deposited: 02 Feb 2015 09:54
Last Modified: 17 Dec 2022 00:49
DOI: 10.17638/02006320