Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging

Hasnain, Samar (2014) Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging. Nature Communications, 5 (1). 3798-.

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Abstract

Nanostructures formed from biological macromolecular complexes utilizing the self-assembly properties of smaller building blocks such as DNA and RNA hold promise for many applications, including sensing and drug delivery. New tools are required for their structural characterization. Intense, femtosecond X-ray pulses from X-ray free-electron lasers enable single-shot imaging allowing for instantaneous views of nanostructures at ambient temperatures. When combined judiciously with synchrotron X-rays of complimentary nature, suitable for observing steady-state features, it is possible to perform ab initio structural investigation. Here we demonstrate a successful combination of femtosecond X-ray single-shot diffraction with an X-ray free-electron laser and coherent diffraction imaging with synchrotron X-rays to provide an insight into the nanostructure formation of a biological macromolecular complex: RNA interference microsponges. This newly introduced multimodal analysis with coherent X-rays can be applied to unveil nano-scale structural motifs from functional nanomaterials or biological nanocomplexes, without requiring a priori knowledge.

Item Type:	Article
Uncontrolled Keywords:	applied optics, free-electron lasers, imaging, RNAi
Subjects:	?? Q1 ??
Depositing User:	Symplectic Admin
Date Deposited:	30 Mar 2015 08:56
Last Modified:	16 Dec 2022 12:32
DOI:	10.1038/ncomms4798
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2009301