A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry

Tamarozzi, Francesca, Wright, Helen L ORCID: 0000-0003-0442-3134, Thomas, Huw B, Edwards, Steven W ORCID: 0000-0002-7074-0552 and Taylor, Mark J (2014) A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry IMMUNOLOGY LETTERS, 162 (2). pp. 194-198. ISSN 0165-2478, 1879-0542

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Abstract

We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

Item Type:	Article
Uncontrolled Keywords:	Neutrophil, Interleukin-17, IL-17A, Wolbachia
Depositing User:	Symplectic Admin
Date Deposited:	14 May 2015 09:42
Last Modified:	16 Jun 2026 10:17
DOI:	10.1016/j.imlet.2014.10.025
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2011303
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