A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry



Tamarozzi, Francesca, Wright, Helen L ORCID: 0000-0003-0442-3134, Thomas, Huw B, Edwards, Steven W ORCID: 0000-0002-7074-0552 and Taylor, Mark J
(2014) A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry. IMMUNOLOGY LETTERS, 162 (2). pp. 194-198.

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Abstract

We identified IL-17A-positive neutrophils in Wolbachia-positive Onchocerca volvulus nodules using an antibody that has previously reported IL-17A-positive neutrophils in several inflammatory conditions. However, we could not detect IL-17A using a range of alternative assays. Our data question the IL-17A antibody specificity and the ability of human neutrophils to express IL-17A.

Item Type: Article
Uncontrolled Keywords: Neutrophil, Interleukin-17, IL-17A, Wolbachia
Depositing User: Symplectic Admin
Date Deposited: 14 May 2015 09:42
Last Modified: 16 Dec 2022 16:48
DOI: 10.1016/j.imlet.2014.10.025
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/2011303

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