Distribution of purinergic P2X receptors in the equine digit, cervical spinal cord and dorsal root ganglia



Zamboulis, DE ORCID: 0000-0003-2839-7620, Senior, JM ORCID: 0000-0002-9425-9261, Clegg, PD ORCID: 0000-0003-0632-0032, Gallagher, JA ORCID: 0000-0002-0852-279X, Carter, SD ORCID: 0000-0002-3585-9400 and Milner, PI
(2013) Distribution of purinergic P2X receptors in the equine digit, cervical spinal cord and dorsal root ganglia. Purinergic Signalling, 9 (3). pp. 383-393.

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Abstract

Purinergic pathways are considered important in pain transmission, and P2X receptors are a key part of this system which has received little attention in the horse. The aim of this study was to identify and characterise the distribution of P2X receptor subtypes in the equine digit and associated vasculature and nervous tissue, including peripheral nerves, dorsal root ganglia and cervical spinal cord, using PCR, Western blot analysis and immunohistochemistry. mRNA signal for most of the tested P2X receptor subunits (P2X1-5, 7) was detected in all sampled equine tissues, whereas P2X6 receptor subunit was predominantly expressed in the dorsal root ganglia and spinal cord. Western blot analysis validated the specificity of P2X1-3, 7 antibodies, and these were used in immunohistochemistry studies. P2X1-3, 7 receptor subunits were found in smooth muscle cells in the palmar digital artery and vein with the exception of the P2X3 subunit that was present only in the vein. However, endothelial cells in the palmar digital artery and vein were positive only for P2X2 and P2X3 receptor subunits. Neurons and nerve fibres in the peripheral and central nervous system were positive for P2X1-3 receptor subunits, whereas glial cells were positive for P2X7 and P2X1 and 2 receptor subunits. This previously unreported distribution of P2X subtypes may suggest important tissue specific roles in physiological and pathological processes.

Item Type: Article
Additional Information: ## TULIP Type: Articles/Papers (Journal) ##
Depositing User: Symplectic Admin
Date Deposited: 20 May 2015 09:48
Last Modified: 17 Dec 2022 01:42
DOI: 10.1007/s11302-013-9356-5
URI: https://livrepository.liverpool.ac.uk/id/eprint/2011542