Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics

Strzelecka, Dominika, Holman, Stephen W and Eyers, Claire E ORCID: 0000-0002-3223-5926 (2015) Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 391. pp. 157-160.

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Abstract

Dimethyl sulfoxide (DMSO) has been advocated as a beneficial additive to electrospray solvents for peptide analysis due to the improved ionisation efficiency conferred. Previous reports have shown that the resultant improvements in peptide ion signal intensities are non-uniform. As a result, it was hypothesised that inclusion of DMSO in electrospray solvents could be detrimental to the outcome of intensity-based label-free absolute quantification approaches, specifically the top 3 method. The effect of DMSO as a mobile phase additive in top 3 label-free quantification was therefore evaluated. We show that inclusion of DMSO enhances data quality, improving the precision and number of proteins quantified, with no significant change to the quantification values observed in its absence.

Item Type:	Article
Uncontrolled Keywords:	Proteomics, Quantification, DMSO, Label-free, Peptide, LC-MS
Depositing User:	Symplectic Admin
Date Deposited:	15 Jul 2015 09:58
Last Modified:	15 Dec 2022 11:41
DOI:	10.1016/j.ijms.2015.07.004
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2016462