Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue



Armstrong, JPK, Shakur, R, Horne, JP, Dickinson, SC, Armstrong, CT, Lau, K, Kadiwala, J, Lowe, R, Seddon, A, Mann, S
et al (show 3 more authors) (2015) Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue. Nature Communications, 6.

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Abstract

Restricted oxygen diffusion can result in central cell necrosis in engineered tissue, a problem that is exacerbated when engineering large tissue constructs for clinical application. Here we show that pre-treating human mesenchymal stem cells (hMSCs) with synthetic membrane-active myoglobin-polymer–surfactant complexes can provide a reservoir of oxygen capable of alleviating necrosis at the centre of hyaline cartilage. This is achieved through the development of a new cell functionalization methodology based on polymer–surfactant conjugation, which allows the delivery of functional proteins to the hMSC membrane. This new approach circumvents the need for cell surface engineering using protein chimerization or genetic transfection, and we demonstrate that the surface-modified hMSCs retain their ability to proliferate and to undergo multilineage differentiation. The functionalization technology is facile, versatile and non-disruptive, and in addition to tissue oxygenation, it should have far-reaching application in a host of tissue engineering and cell-based therapies.

Item Type: Article
Uncontrolled Keywords: Biomaterials – cells, Mesenchymal stem cells, Tissue engineering
Subjects: ?? Q1 ??
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Depositing User: Symplectic Admin
Date Deposited: 15 Jul 2015 08:24
Last Modified: 29 Nov 2021 08:11
DOI: 10.1038/ncomms8405
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/2016482