Elucidating mechanisms of carbamate resistance and carbamate/pyrethroid cross resistance in An. funestus in Africa



Ndula, Miranda
Elucidating mechanisms of carbamate resistance and carbamate/pyrethroid cross resistance in An. funestus in Africa. PhD thesis, University of Liverpool.

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Abstract

Malaria remains one of the most debilitating tropical diseases with more than 90% of cases in Africa among children under five and pregnant women. Resistance observed against the main insecticides used in public health sector in major vectors such as Anopheles funestus is threatening the success of vector control interventions. To improve the design of suitable resistance management strategies, it is crucial to elucidate the underlining molecular basis of resistance or cross-resistance between insecticides and also establish patterns of gene flow between populations to predict the speed and direction of spread of resistance genes. To address these questions, this study has investigated the molecular basis of resistance to carbamates and cross/resistance to carbamates/pyrethroids in a population of An. funestus from Malawi. This study has revealed that metabolic resistance is the main mechanism driving carbamate resistance through the over-expression of Cytochrome P450 genes. Genome-wide microarray-based transcription analyses consistently revealed that the duplicated P450 genes CYP6P9a and CYP6P9b were among the most up-regulated genes (>2-fold; P

Item Type: Thesis (PhD)
Additional Information: Date: 2014 (completed)
Subjects: ?? RZ ??
Depositing User: Symplectic Admin
Date Deposited: 15 Jan 2016 16:24
Last Modified: 17 Dec 2022 01:54
DOI: 10.17638/02027219
URI: https://livrepository.liverpool.ac.uk/id/eprint/2027219