Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer


Liu, X, Li, J, Dong, J ORCID: 0000-0002-4119-8039, Hu, C, Gong, W and Wang, J (2012) Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer Angewandte Chemie International Edition, 51 (41). pp. 10261-10265. ISSN 1433-7851, 1521-3773

[thumbnail of abstract] Text
abstract - Unspecified
Access to this file is embargoed until Unspecified.
Download (58kB)
[thumbnail of abstract] Text
abstract - Unspecified
Access to this file is embargoed until Unspecified.
Download (45kB)
[thumbnail of 22936654] Text
22936654 - Unspecified
Access to this file is embargoed until Unspecified.
Download (76kB)

Abstract

ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu <sup>II</sup> ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu<sup>II</sup> ions. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Item Type: Article
Uncontrolled Keywords: Animals, Scyphozoa, Metals, Amino Acids, Tyrosine, Green Fluorescent Proteins, Chelating Agents, Protein Engineering, Binding Sites, Protein Structure, Tertiary, Electron Transport
Subjects: ?? QD ??
Depositing User: Symplectic Admin
Date Deposited: 26 Nov 2015 16:27
Last Modified: 24 Jan 2026 00:27
DOI: 10.1002/anie.201204962
Related Websites:
URI: https://livrepository.liverpool.ac.uk/id/eprint/2039304
Disclaimer: The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate.
Altmetric
CORE (COnnecting REpositories)