Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer.



Liu, Xiaohong, Li, Jiasong ORCID: 0000-0003-0341-2038, Dong, Jianshu ORCID: 0000-0002-4119-8039, Hu, Cheng, Gong, Weimin and Wang, Jiangyun
(2012) Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer. Angewandte Chemie (International ed. in English), 51 (41). pp. 10261-10265.

[img] Text
abstract - Unspecified
Access to this file is embargoed until Unspecified.

Download (58kB)
[img] Text
abstract - Unspecified
Access to this file is embargoed until Unspecified.

Download (45kB)
[img] Text
22936654 - Unspecified
Access to this file is embargoed until Unspecified.

Download (76kB)

Abstract

ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu II ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to CuII ions. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Item Type: Article
Uncontrolled Keywords: Animals, Scyphozoa, Metals, Amino Acids, Tyrosine, Green Fluorescent Proteins, Chelating Agents, Protein Engineering, Binding Sites, Protein Structure, Tertiary, Electron Transport
Subjects: ?? QD ??
Depositing User: Symplectic Admin
Date Deposited: 26 Nov 2015 16:27
Last Modified: 17 Dec 2022 01:33
DOI: 10.1002/anie.201204962
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/2039304