Comparison of Binax NOW urine antigen test and pneumococcal DNA assay using qPCR before and after nasopharyngeal swabbing in healthy Malawian children.

Lees, EA, Ho, DKK, Guiver, M, Mankhambo, LA, French, N ORCID: 0000-0003-4814-8293 and Carrol, ED ORCID: 0000-0001-8357-7726 (2015) Comparison of Binax NOW urine antigen test and pneumococcal DNA assay using qPCR before and after nasopharyngeal swabbing in healthy Malawian children. New microbes and new infections, 8. pp. 4-6.

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Abstract

Diagnosis of invasive pneumococcal disease is challenging. We compared Binax NOW pneumococcal urinary antigen test with blood pneumococcal PCR in healthy Malawian children with and without pneumococcal carriage, and we found a high false-positive rate with Binax NOW. Blood pneumococcal PCR positivity was 66/88 (75%) compared to 5/27 (18%) when nasopharyngeal swabbing was performed first compared to after blood sampling for pneumococcal blood PCR. We speculate that nasopharyngeal swabbing may be causing a breach of mucosal integrity, leading to invasion into the bloodstream. These findings need to be confirmed with autolysin-based PCR assays.

Item Type:	Article
Uncontrolled Keywords:	Binax NOW urine antigen test, DNAaemia, invasive pneumococcal disease, nasopharyngeal swabbing, pneumococcal PCR
Subjects:	?? RJ ??
Depositing User:	Symplectic Admin
Date Deposited:	01 Dec 2015 15:02
Last Modified:	17 Dec 2022 00:49
DOI:	10.1016/j.nmni.2015.08.001
Publisher's Statement :	© 2015 The Authors. Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2040159