Evaluation of the role of polyelectrolyte deposition conditions in growth factor release

Peterson, AM, Pilz-Allen, C, Moehwald, H and Shchukin, DG ORCID: 0000-0002-2936-804X (2014) Evaluation of the role of polyelectrolyte deposition conditions in growth factor release. JOURNAL OF MATERIALS CHEMISTRY B, 2 (18). pp. 2680-2687.

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Abstract

Polyelectrolyte multilayer coatings were prepared from solutions of poly(methacrylic acid) and poly-l-histidine. Bone morphogenetic protein 2 (BMP-2) was adsorbed onto the surface of anodized titanium and polyelectrolyte multilayer coatings were built up on top of the BMP-2. The effect of deposition conditions on coating properties and preosteoblast response was measured by comparing coatings prepared under natural conditions to those prepared from solutions at pH = 6.0 and solutions containing 0.1 M NaCl. High levels of BMP-2 release were achieved, with coatings prepared from pH = 6.0 solutions releasing 86 ng cm^-2 and coatings prepared from solutions containing 0.1 M NaCl releasing 114 ng cm^-2 over 25 days. Enhanced preosteoblast differentiation was observed on coatings prepared from modified solutions; however, this increased differentiation was apparent for BMP-2-eluting and control coatings. Additionally, a positive relationship between surface roughness and differentiation was observed, which may account for increased differentiation for systems that do not release BMP-2.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Subjects:	?? QD ??
Depositing User:	Symplectic Admin
Date Deposited:	11 Jan 2016 11:31
Last Modified:	25 Jan 2024 00:18
DOI:	10.1039/c3tb21757d
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2045739

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