Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population

Bennett, Aisleen, Bar-Zeev, Naor ORCID: 0000-0003-0570-4624, Jere, Khuzwayo ORCID: 0000-0003-3376-8529, Tate, JE, Parashar, UD, Nakagomi, Osamu, Heyderman, Robert, French, Neil ORCID: 0000-0003-4814-8293, Iturriza-Gomara, Miren ORCID: 0000-0001-5816-6423 and Cunliffe, Nigel ORCID: 0000-0002-5449-4988 (2015) Determination of a Viral Load Threshold To Distinguish Symptomatic versus Asymptomatic Rotavirus Infection in a High-Disease-Burden African Population. Journal of Clinical Microbiology, 53 (6). pp. 1951-1954.

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Official URL: https://jcm.asm.org/content/53/6/1951/article-info

Abstract

We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.

Item Type:	Article
Additional Information:	Cite as: Bennett A, Bar-Zeev N, Jere KC, Tate JE, Parashar UD, Nakagomi O, Heyderman RS, French N, Iturriza-Gomara M, Cunliffe NA. 2015. Determination of a viral load threshold to distinguish symptomatic versus asymptomatic rotavirus infection in a high-disease-burden African population. J Clin Microbiol 53:1951–1954. doi:10.1128/JCM.00875-15.
Uncontrolled Keywords:	Humans, Rotavirus, Rotavirus Infections, Gastroenteritis, Immunoenzyme Techniques, Viral Load, Child, Preschool, Infant, Infant, Newborn, Malawi, Asymptomatic Infections, Real-Time Polymerase Chain Reaction
Depositing User:	Symplectic Admin
Date Deposited:	12 Jan 2016 17:03
Last Modified:	15 Dec 2022 23:52
DOI:	10.1128/JCM.00875-15
Publisher's Statement :	Copyright © 2015, Bennett et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/2046299