Stem Cells Derived from Neonatal Mouse Kidney Generate Functional Proximal Tubule-Like Cells and Integrate into Developing Nephrons <i>In Vitro</i>



Ranghini, Egon, Mora, Cristina Fuente, Edgar, David, Kenny, Simon E, Murray, Patricia ORCID: 0000-0003-1316-148X and Wilm, Bettina
(2013) Stem Cells Derived from Neonatal Mouse Kidney Generate Functional Proximal Tubule-Like Cells and Integrate into Developing Nephrons <i>In Vitro</i>. PLOS ONE, 8 (5). e62953-.

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Abstract

We have recently shown that kidney-derived stem cells (KSCs) isolated from the mouse newborn kidney differentiate into a range of kidney-specific cell types. However, the functionality and integration capacity of these mouse KSCs remain unknown. Therefore, the main objectives of this study were (1) to determine if proximal tubule-like cells, generated in vitro from KSCs, displayed absorptive function typical of proximal tubule cells in vivo, and (2) to establish whether the ability of KSCs to integrate into developing nephrons was comparable with that of metanephric mesenchyme (MM), a transient population of progenitor cells that gives rise to the nephrons during kidney organogenesis. We found that proximal tubule-like cells generated in vitro from mouse KSCs displayed megalin-dependent absorptive function. Subsequently, we used a chimeric kidney rudiment culture system to show that the KSCs could generate proximal tubule cells and podocytes that were appropriately located within the developing nephrons. Finally, we compared the ability of KSCs to integrate into developing kidneys ex vivo with that of metanephric mesenchyme cells. We found that KSCs integrated into nascent nephrons to a similar extent as metanephric mesenchyme cells while both were excluded from ureteric bud branches. Our analysis of the behavior of the two cell types shows that some, but not all KSC characteristics are similar to those of the MM.

Item Type: Article
Additional Information: ## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords: Kidney Tubules, Proximal, Stem Cells, Mesoderm, Animals, Animals, Newborn, Mice, Hybridization, Genetic, Cell Differentiation, Absorption, Podocytes
Subjects: ?? Q1 ??
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Depositing User: Symplectic Admin
Date Deposited: 15 Jan 2016 10:30
Last Modified: 19 Oct 2023 08:50
DOI: 10.1371/journal.pone.0062953
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/2046939