Cross-species proteomics in analysis of mammalian sperm proteins

Bayram, Helen, Claydon, Amy, Brownridge, Philip, Hurst, Jane ORCID: 0000-0002-3728-9624, Mileham, Alan, Stockley, Paula, Beynon, Robert ORCID: 0000-0003-0857-495X and Hammond, Dean ORCID: 0000-0002-6326-8739
(2016) Cross-species proteomics in analysis of mammalian sperm proteins. Journal of Proteomics, 135. pp. 38-50.

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Many proteomics studies are conducted in model organisms for which fully annotated, detailed, high quality proteomes are available. By contrast, many studies in ecology and evolution are conducted in species which lack high quality proteome data, limiting the perceived value of a proteomic approach for protein discovery and quantification. This is particularly true of rapidly evolving proteins in the reproductive system, such as those that have an immune function or are under sexual selection, and can compromise the potential for cross-species proteomics to yield confident identification. In this investigation we analysed the sperm proteome, from a range of ungulates and rodents, and explored the potential of routine proteomics workflows to yield characterisation and quantification in non-model organisms. We report that database searching is robust to cross-species matching for a mammalian core sperm proteome, comprising 623 proteins that were common to most of the 19 species studied here, suggesting that these proteins are likely to be present and identifiable across many mammalian sperm. Further, label-free quantification reveals a consistent pattern of expression level. Functional analysis of this core proteome suggests consistency with previous studies limited to model organisms and has value as a quantitative reference for analysis of species-specific protein characterisation.

Item Type: Article
Uncontrolled Keywords: Cross-species proteomics, Sperm, Label-free quantification
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Depositing User: Symplectic Admin
Date Deposited: 25 Jan 2016 11:03
Last Modified: 16 Dec 2022 16:02
DOI: 10.1016/j.jprot.2015.12.027
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