Molecular Epidemiology and Characterization of Campylobacter spp. Isolated from Wild Bird Populations in Northern England



Hughes, Laura A, Bennett, Malcolm, Coffey, Peter, Elliott, John, Jones, Trevor R, Jones, Richard C, Lahuerta-Marin, Angela, Leatherbarrow, A Howard, McNiffe, Kenny, Norman, David
et al (show 2 more authors) (2009) Molecular Epidemiology and Characterization of Campylobacter spp. Isolated from Wild Bird Populations in Northern England. Applied and Environmental Microbiology, 75 (10). 3007 - 3015.

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Abstract

<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Campylobacter</jats:italic> infections have been reported at prevalences ranging from 2 to 50% in a range of wild bird species, although there have been few studies that have investigated the molecular epidemiology of <jats:italic>Campylobacter</jats:italic> spp. Consequently, whether wild birds are a source of infection in humans or domestic livestock or are mainly recipients of domestic animal strains and whether separate cycles of infection occur remain unknown. To address these questions, serial cross-sectional surveys of wild bird populations in northern England were carried out over a 2-year period. Fecal samples were collected from 2,084 wild bird individuals and screened for the presence of <jats:italic>Campylobacter</jats:italic> spp. A total of 56 isolates were recovered from 29 birds sampled at 15 of 167 diverse locales. <jats:italic>Campylobacter jejuni</jats:italic>, <jats:italic>Campylobacter lari</jats:italic>, and <jats:italic>Campylobacter coli</jats:italic> were detected by PCR, and the prevalences of different <jats:italic>Campylobacter</jats:italic> spp. in different avian families ranged from 0% to 33%. Characterization of 36 <jats:italic>C. jejuni</jats:italic> isolates by multilocus sequence typing revealed that wild birds carry both livestock-associated and unique strains of <jats:italic>C. jejuni</jats:italic>. However, the apparent absence of unique wild bird strains of <jats:italic>C. jejuni</jats:italic> in livestock suggests that the direction of infection is predominantly from livestock to wild birds. <jats:italic>C. lari</jats:italic> was detected mainly in wild birds sampled in an estuarine or coastal habitat. Fifteen <jats:italic>C. lari</jats:italic> isolates were analyzed by macrorestriction pulsed-field gel electrophoresis, which revealed genetically diverse populations of <jats:italic>C. lari</jats:italic> in Eurasian oystercatchers (<jats:italic>Haematopus ostralegus</jats:italic>) and clonal populations in magpies (<jats:italic>Pica pica</jats:italic>).</jats:p>

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 11 Apr 2016 09:56
Last Modified: 16 Sep 2020 08:47
DOI: 10.1128/aem.02458-08
URI: http://livrepository.liverpool.ac.uk/id/eprint/3000136