The role of soluble plant fibres (non-starch polysaccharides, NSP) in the maintenance of intestinal health and prevention of diarrhoeal disease

Simpson, H (2016) The role of soluble plant fibres (non-starch polysaccharides, NSP) in the maintenance of intestinal health and prevention of diarrhoeal disease PhD thesis, University of Liverpool.

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Abstract

It has long been proposed that a high intake of dietary fibre promotes good intestinal health. Work performed previously by our research group suggests that soluble dietary fibre might have a particularly beneficial impact on intestinal health via its ability to inhibit potentially harmful interactions between bacterial pathogens and the gut epithelium. The aims of this thesis were to evaluate soluble plantain NSP for its ability to disrupt the epithelial interactions of diarrhoeal pathogens C. difficile and Enterotoxigenic Escherichia coli (ETEC), as well as other bacterial components implicated in diarrhoeal disease. Work was also performed to characterise the specific inhibitory fraction of plantain NSP, and in addition, to establish the molecular mechanism underlying its inhibitory activity. A range of soluble dietary fibres were shown to significantly inhibit the in vitro epithelial adhesion of C. difficile and ETEC, but out of all the fibres tested, soluble plantain NSP exhibited the highest efficacy. Plantain NSP also significantly inhibited the epithelial adhesion of eleven C. difficile clinical isolates, irrespective of their toxin expression or ribotype status. Furthermore, plantain NSP blocked the ... (continues)

Item Type:	Thesis (PhD)
Divisions:	Faculty of Health & Life Sciences
Depositing User:	Symplectic Admin
Date Deposited:	01 Aug 2016 09:48
Last Modified:	31 Oct 2024 07:01
DOI:	10.17638/03001329
Supervisors:	Campbell, BJ ORCID: 0000-0002-7407-012X Rhodes, JM ORCID: 0000-0002-1302-260X
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3001329
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