Unique microbial-derived volatile organic compounds in portal venous circulation in murine non-alcoholic fatty liver disease

Collapse authors list.
Reid, DT, McDonald, B, Khalid, T, Vo, T, Schenck, LP, Surette, MG, Beck, PL, Reimer, RA, Probert, CS ORCID: 0000-0003-0477-6714, Rioux, KP et al (show 1 more authors) and Eksteen, B (2016) Unique microbial-derived volatile organic compounds in portal venous circulation in murine non-alcoholic fatty liver disease BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1862 (7). pp. 1337-1344. ISSN 0925-4439, 1879-260X

Text Reid et al_MBD 2016_all_ChrisP.doc - Author Accepted Manuscript Download (36MB)

Abstract

Background and aims: Non-alcoholic fatty liver disease is now the leading liver disease in North America. The progression of non-alcoholic fatty liver disease to the inflammatory condition, non-alcoholic steatohepatitis is complex and currently not well understood. Intestinal microbial dysbiosis has been implicated in the development of non-alcoholic fatty liver disease and progression of non-alcoholic steatohepatitis. Volatile organic compounds are byproducts of microbial metabolism in the gut that may enter portal circulation and have hepatotoxic effects contributing to the pathogenesis of non-alcoholic steatohepatitis. To test this hypothesis, we measured volatile organic compounds in cecal luminal contents and portal venous blood in a mouse model of non-alcoholic steatohepatitis. Methods: Gas chromatography-mass spectrometry analysis was conducted on cecal content and portal vein blood for volatile organic compound detection from mice fed a methionine and choline deficient diet, which induces non-alcoholic steatohepatitis. The colonic microbiome was studied by 16S rRNA gene amplification using the Illumina MiSeq platform. Results: Sixty-eight volatile organic compounds were detected in cecal luminal content, a subset of which was also present in portal venous blood. Importantly, differences in portal venous volatile organic compounds were associated with diet-induced steatohepatitis establishing a biochemical link between gut microbiota-derived volatile organic compounds and increased susceptibility to non-alcoholic steatohepatitis. Conclusion: Our model creates a novel tool to further study the role of gut-derived volatile organic compounds in the pathogenesis of non-alcoholic steatohepatitis.

Item Type:	Article
Uncontrolled Keywords:	Volatile organic compounds, Liver, Microbiome, Metabolomics
Depositing User:	Symplectic Admin
Date Deposited:	09 Nov 2016 08:49
Last Modified:	16 Jun 2026 09:51
DOI:	10.1016/j.bbadis.2016.04.005
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3001580
Disclaimer:	The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate.