Reid, DT, McDonald, B, Khalid, T, Vo, T, Schenck, LP, Surette, MG, Beck, PL, Reimer, RA, Probert, CS ORCID: 0000-0003-4550-0239, Rioux, KP et al (show 1 more authors)
(2016)
Unique microbial-derived volatile organic compounds in portal venous circulation in murine non-alcoholic fatty liver disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1862 (7).
pp. 1337-1344.
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Reid et al_MBD 2016_all_ChrisP.doc - Author Accepted Manuscript Download (36MB) |
Abstract
<h4>Background and aims</h4>Non-alcoholic fatty liver disease is now the leading liver disease in North America. The progression of non-alcoholic fatty liver disease to the inflammatory condition, non-alcoholic steatohepatitis is complex and currently not well understood. Intestinal microbial dysbiosis has been implicated in the development of non-alcoholic fatty liver disease and progression of non-alcoholic steatohepatitis. Volatile organic compounds are byproducts of microbial metabolism in the gut that may enter portal circulation and have hepatotoxic effects contributing to the pathogenesis of non-alcoholic steatohepatitis. To test this hypothesis, we measured volatile organic compounds in cecal luminal contents and portal venous blood in a mouse model of non-alcoholic steatohepatitis.<h4>Methods</h4>Gas chromatography-mass spectrometry analysis was conducted on cecal content and portal vein blood for volatile organic compound detection from mice fed a methionine and choline deficient diet, which induces non-alcoholic steatohepatitis. The colonic microbiome was studied by 16S rRNA gene amplification using the Illumina MiSeq platform.<h4>Results</h4>Sixty-eight volatile organic compounds were detected in cecal luminal content, a subset of which was also present in portal venous blood. Importantly, differences in portal venous volatile organic compounds were associated with diet-induced steatohepatitis establishing a biochemical link between gut microbiota-derived volatile organic compounds and increased susceptibility to non-alcoholic steatohepatitis.<h4>Conclusion</h4>Our model creates a novel tool to further study the role of gut-derived volatile organic compounds in the pathogenesis of non-alcoholic steatohepatitis.
Item Type: | Article |
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Uncontrolled Keywords: | Volatile organic compounds, Liver, Microbiome, Metabolomics |
Depositing User: | Symplectic Admin |
Date Deposited: | 09 Nov 2016 08:49 |
Last Modified: | 19 Jan 2023 07:36 |
DOI: | 10.1016/j.bbadis.2016.04.005 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3001580 |