Potential DNA binding and nuclease functions of ComEC domains characterised in silico



Baker, JA, Simkovic, F ORCID: 0000-0003-3430-6828, Taylor, HMC and Rigden, DJ ORCID: 0000-0002-7565-8937
(2016) Potential DNA binding and nuclease functions of ComEC domains characterised in silico. Proteins: Structure, Function, and Bioinformatics, 84. 1431 - 1442.

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Abstract

Bacterial competence, which can be natural or induced, allows the uptake of exogenous double stranded DNA (dsDNA) into a competent bacterium. This process is known as transformation. A multiprotein assembly binds and processes the dsDNA to import one strand and degrade another yet the underlying molecular mechanisms are relatively poorly understood. Here distant relationships of domains in Competence protein EC (ComEC) of Bacillus subtilis (Uniprot: P39695) were characterized. DNA‐protein interactions were investigated in silico by analyzing models for structural conservation, surface electrostatics and structure‐based DNA binding propensity; and by data‐driven macromolecular docking of DNA to models. Our findings suggest that the DUF4131 domain contains a cryptic DNA‐binding OB fold domain and that the β‐lactamase‐like domain is the hitherto cryptic competence nuclease. Proteins 2016; 84:1431–1442. © 2016 The Authors Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc.

Item Type: Article
Uncontrolled Keywords: bacterial competence, ComEC, evolutionary covariance, domain structure, protein modeling
Depositing User: Symplectic Admin
Date Deposited: 14 Jun 2016 14:11
Last Modified: 24 Jun 2021 11:03
DOI: 10.1002/prot.25088
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3001670