PERP, a host tetraspanning membrane protein, is required for Salmonella-induced inflammation

Hallstrom, KN, Srikanth, CV, Agbor, TA, Dumont, CM, Peters, KN, Paraoan, L ORCID: 0000-0001-7568-7116, Casanova, JE, Boll, EJ and McCormick, BA
(2015) PERP, a host tetraspanning membrane protein, is required for Salmonella-induced inflammation. Cellular Microbiology, 17 (6). pp. 843-859.

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Salmonella enterica Typhimurium induces intestinal inflammation through the activity of type III secreted effector (T3SE) proteins. Our prior results indicate that the secretion of the T3SE SipA and the ability of SipA to induce epithelial cell responses that lead to induction of polymorphonuclear transepithelial migration are not coupled to its direct delivery into epithelial cells from Salmonella. We therefore tested the hypothesis that SipA interacts with a membrane protein located at the apical surface of intestinal epithelial cells. Employing a split ubiquitin yeast-two-hybrid screen, we identified the tetraspanning membrane protein, p53 effector related to PMP-22 (PERP), as a SipA binding partner. SipA and PERP appear to have intersecting activities as we found PERP to be involved in proinflammatory pathways shown to be regulated by SipA. In sum, our studies reveal a critical role for PERP in the pathogenesis of S. Typhimurium, and for the first time demonstrate that SipA, a T3SE protein, can engage a host protein at the epithelial surface.

Item Type: Article
Uncontrolled Keywords: Cell Line, Epithelial Cells, Humans, Salmonella typhimurium, Inflammation, Microfilament Proteins, Bacterial Proteins, Membrane Proteins, Two-Hybrid System Techniques, Protein Interaction Mapping, Protein Binding, Genes, Tumor Suppressor, Host-Pathogen Interactions, Transendothelial and Transepithelial Migration
Depositing User: Symplectic Admin
Date Deposited: 04 Jul 2016 08:31
Last Modified: 19 Jan 2023 07:34
DOI: 10.1111/cmi.12406
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