Positional signaling and expression of ENHANCER OF TRY AND CPC1 are tuned to increase root hair density in response to phosphate deficiency in Arabidopsis thaliana.



Savage, Natasha ORCID: 0000-0002-6831-6533, Yang, Thomas JW, Chen, Chung Ying, Lin, Kai-Lan, Monk, Nicholas AM ORCID: 0000-0002-5465-4857 and Schmidt, Wolfgang
(2013) Positional signaling and expression of ENHANCER OF TRY AND CPC1 are tuned to increase root hair density in response to phosphate deficiency in Arabidopsis thaliana. PloS one, 8 (10). e75452 - ?.

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Abstract

Phosphate (Pi) deficiency induces a multitude of responses aimed at improving the acquisition of Pi, including an increased density of root hairs. To understand the mechanisms involved in Pi deficiency-induced alterations of the root hair phenotype in Arabidopsis (Arabidopsis thaliana), we analyzed the patterning and length of root epidermal cells under control and Pi-deficient conditions in wild-type plants and in four mutants defective in the expression of master regulators of cell fate, CAPRICE (CPC), ENHANCER OF TRY AND CPC 1 (ETC1), WEREWOLF (WER) and SCRAMBLED (SCM). From this analysis we deduced that the longitudinal cell length of root epidermal cells is dependent on the correct perception of a positional signal ('cortical bias') in both control and Pi-deficient plants; mutants defective in the receptor of the signal, SCM, produced short cells characteristic of root hair-forming cells (trichoblasts). Simulating the effect of cortical bias on the time-evolving probability of cell fate supports a scenario in which a compromised positional signal delays the time point at which non-hair cells opt out the default trichoblast pathway, resulting in short, trichoblast-like non-hair cells. Collectively, our data show that Pi-deficient plants increase root hair density by the formation of shorter cells, resulting in a higher frequency of hairs per unit root length, and additional trichoblast cell fate assignment via increased expression of ETC1.

Item Type: Article
Uncontrolled Keywords: Plants, Genetically Modified, Arabidopsis, Plant Roots, Phosphates, Arabidopsis Proteins, Signal Transduction, Gene Expression Regulation, Plant
Depositing User: Symplectic Admin
Date Deposited: 07 Apr 2017 13:18
Last Modified: 08 May 2020 17:18
DOI: 10.1371/journal.pone.0075452
URI: http://livrepository.liverpool.ac.uk/id/eprint/3002235