Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL



Halliwell, DE, Morais, CLM, Lima, KMG, Trevisan, J, Siggel-King, MRF, Craig, T, Ingham, J, Martin, DS, Heys, KA, Kyrgiou, M
et al (show 7 more authors) (2016) Imaging cervical cytology with scanning near-field optical microscopy (SNOM) coupled with an IR-FEL. Scientific Reports.

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Abstract

Cervical cancer remains a major cause of morbidity and mortality among women, especially in the developing world. Increased synthesis of proteins, lipids and nucleic acids is a pre-condition for the rapid proliferation of cancer cells. We show that scanning near-field optical microscopy, in combination with an infrared free electron laser (SNOM-IR-FEL), is able to distinguish between normal and squamous low-grade and high-grade dyskaryosis and between normal and mixed squamous/glandular pre-invasive and adenocarcinoma cervical lesions, at designated wavelengths associated with DNA, Amide I/II and lipids. These findings evidence the promise of the SNOM-IR-FEL technique in obtaining chemical information relevant to the detection of cervical cell abnormalities and cancer diagnosis at spatial resolutions below the diffraction limit (≥0.2 μm). We compare these results with analyses following attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy; although this latter approach has been demonstrated to detect underlying cervical atypia missed by conventional cytology, it is limited by a spatial resolution of ~3 μm to 30 μm due to the optical diffraction limit.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 15 Jul 2016 09:25
Last Modified: 09 Jan 2021 08:20
DOI: 10.1038/srep29494
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3002349