Dynamic NF-κb and E2F interactions control the priority and timing of inflammatory signalling and cell proliferation



Ankers, JM, Awais, R, Jones, NA, Boyd, J ORCID: 0000-0003-0858-7179, Ryan, S, Adamson, AD, Harper, CV, Bridge, L, Spiller, DG, Jackson, DA
et al (show 3 more authors) (2016) Dynamic NF-κb and E2F interactions control the priority and timing of inflammatory signalling and cell proliferation. eLife, 2016 (5).

[img] Text
15-03-16_Ankers et.al Final.docx - Accepted Version

Download (1MB)
[img] Atom XML (admin)
2017-05-12T11:30:46Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T12:26:57Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T13:26:10Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T14:26:18Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T15:26:22Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T16:26:58Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T17:26:13Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T18:27:07Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T19:26:44Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T20:27:06Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T21:27:12Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T22:28:17Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T23:34:00Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T01:29:00Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T02:28:01Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T03:28:15Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T04:51:49Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T05:28:06Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T06:27:10Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T07:27:27Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T08:27:57Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T09:27:07Z.atom - Unspecified

Download (0B)

Abstract

© Ankers et al.Dynamic cellular systems reprogram gene expression to ensure appropriate cellular fate responses to specific extracellular cues. Here we demonstrate that the dynamics of Nuclear Factor kappa B (NF-κB) signalling and the cell cycle are prioritised differently depending on the timing of an inflammatory signal. Using iterative experimental and computational analyses, we show physical and functional interactions between NF-κB and the E2 Factor 1 (E2F-1) and E2 Factor 4 (E2F-4) cell cycle regulators. These interactions modulate the NF-κB response. In S-phase, the NF-κB response was delayed or repressed, while cell cycle progression was unimpeded. By contrast, activation of NF-κB at the G1/S boundary resulted in a longer cell cycle and more synchronous initial NF-κB responses between cells. These data identify new mechanisms by which the cellular response to stress is differentially controlled at different stages of the cell cycle.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 19 Jul 2016 13:57
Last Modified: 18 Oct 2021 23:10
DOI: 10.7554/eLife.10473
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3002439