Gas-phase intermolecular phosphate transfer within a phosphohistidine phosphopeptide dimer

Gonzalez-Sanchez, Maria-Belen, Lanucara, Francesco, Hardman, Gemma E and Eyers, Claire E ORCID: 0000-0002-3223-5926
(2014) Gas-phase intermolecular phosphate transfer within a phosphohistidine phosphopeptide dimer. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 367. pp. 28-34.

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The hydrogen bonds and electrostatic interactions that form between the protonated side chain of a basic residue and the negatively charged phosphate of a phosphopeptide can play crucial roles in governing their dissociation pathways under low-energy collision-induced dissociation (CID). Understanding how phosphoramidate (i.e. phosphohistidine, phospholysine and phosphoarginine), rather than phosphomonoester-containing peptides behave during CID is paramount in investigation of these problematic species by tandem mass spectrometry. To this end, a synthetic peptide containing either phosphohistidine (pHis) or phospholysine (pLys) was analyzed by ESI-MS using a Paul-type ion trap (AmaZon, Bruker) and by traveling wave ion mobility-mass spectrometry (Synapt G2-S<i>i</i>, Waters). Analysis of the products of low-energy CID demonstrated formation of a doubly 'phosphorylated' product ion arising from intermolecular gas-phase phosphate transfer within a phosphopeptide dimer. The results are explained by the formation of a homodimeric phosphohistidine (pHis) peptide non-covalent complex (NCX), likely stabilized by the electrostatic interaction between the pHis phosphate group and the protonated <i>C</i>-terminal lysine residue of the peptide. To the best of our knowledge this is the first report of intermolecular gas-phase phosphate transfer from one phosphopeptide to another, leading to a doubly phosphorylated peptide product ion.

Item Type: Article
Additional Information: ## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords: CID, Phosphotransfer, Phosphoramidate, Histidine phosphorylation, Gas-phase dimer, Non-covalent interactions
Depositing User: Symplectic Admin
Date Deposited: 10 Aug 2016 07:30
Last Modified: 19 Jan 2023 07:32
DOI: 10.1016/j.ijms.2014.04.015
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