The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice.



Mitchell, Sharon E, Delville, Camille, Konstantopedos, Penelope, Hurst, Jane ORCID: 0000-0002-3728-9624, Derous, Davina ORCID: 0000-0002-9041-4607, Green, Cara, Chen, Luonan, Han, Jackie JD, Wang, Yingchun, Promislow, Daniel EL ORCID: 0000-0001-7088-4495
et al (show 3 more authors) (2015) The effects of graded levels of calorie restriction: II. Impact of short term calorie and protein restriction on circulating hormone levels, glucose homeostasis and oxidative stress in male C57BL/6 mice. Oncotarget, 6 (27). pp. 23213-23237.

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Abstract

Limiting food intake attenuates many of the deleterious effects of aging, impacting upon healthspan and leading to an increased lifespan. Whether it is the overall restriction of calories (calorie restriction: CR) or the incidental reduction in macronutrients such as protein (protein restriction: PR) that mediate these effects is unclear. The impact of 3 month CR or PR, (10 to 40%), on C57BL/6 mice was compared to controls fed ad libitum. Reductions in circulating leptin, tumor necrosis factor-α and insulin-like growth factor-1 (IGF-1) were relative to the level of CR and individually associated with morphological changes but remained unchanged following PR. Glucose tolerance and insulin sensitivity were improved following CR but not affected by PR. There was no indication that CR had an effect on oxidative damage, however CR lowered antioxidant activity. No biomarkers of oxidative stress were altered by PR. CR significantly reduced levels of major urinary proteins suggesting lowered investment in reproduction. Results here support the idea that reduced adipokine levels, improved insulin/IGF-1 signaling and reduced reproductive investment play important roles in the beneficial effects of CR while, in the short-term, attenuation of oxidative damage is not applicable. None of the positive effects were replicated with PR.

Item Type: Article
Uncontrolled Keywords: Liver, Animals, Mice, Inbred C57BL, Mice, Oxygen, Insulin, Leptin, Glucose, Blood Glucose, Insulin-Like Growth Factor I, Tumor Necrosis Factor-alpha, Dietary Proteins, Antioxidants, Glucose Tolerance Test, Caloric Restriction, Oxidative Stress, Homeostasis, Female, Male, Adipokines
Depositing User: Symplectic Admin
Date Deposited: 13 Sep 2016 09:10
Last Modified: 19 Jan 2023 07:30
DOI: 10.18632/oncotarget.4003
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3003264