Multifunctional Fe₃O₄ nanoparticles for targeted bi-modal imaging of pancreatic cancer

Olariu, Cristina I, Yiu, Humphrey HP, Bouffier, Laurent, Nedjadi, Taoufik, Costello, Eithne, Williams, Steve R, Halloran, Christopher M ORCID: 0000-0002-5471-4178 and Rosseinsky, Matthew J ORCID: 0000-0002-1910-2483 (2011) Multifunctional Fe₃O₄ nanoparticles for targeted bi-modal imaging of pancreatic cancer JOURNAL OF MATERIALS CHEMISTRY, 21 (34). pp. 12650-12659. ISSN 0959-9428, 1364-5501

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Abstract

Amine and carboxylic acid-bifunctionalized iron oxide nanoparticles with robust silane linkages to the nanoparticle surface were prepared with a versatile direct grafting protocol. The contrast in chemistry of these two groups was highlighted by attaching a fluorophore, Rhodamine B isothiocyanate (RITC) onto the amine group and an antibody (EPCAM - epithelial cell adhesion molecule) onto the carboxylic acid groups. The iron oxide core and the RITC tags provide the MRI-fluorescent bi-modal imaging capability. The EPCAM antibody is specific to a protein ubiquitously expressed on the epithelial cell surface. These bifunctionalized nanoparticles target and then undergo facilitated uptake into pancreatic cancer cells (Panc-1) in a time course-monitored controlled study. The integrated optical imaging properties of these magnetic nanoparticles were utilized to monitor the interaction of the nanoparticles with the EPCAM receptors on the cell membrane of the Panc-1 cells. The time-course of the uptake for the targeted and the control particles by the cells was followed allowing the localization within the cell and the impact of particle functionalization to be identified. This system is a candidate for further development as a multi-modular imaging, diagnostic and delivery tool. © 2011 The Royal Society of Chemistry.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	3206 Medical Biotechnology, 40 Engineering, 32 Biomedical and Clinical Sciences, 4018 Nanotechnology, Pancreatic Cancer, Cancer, Rare Diseases, Biomedical Imaging, Biotechnology, Digestive Diseases, Bioengineering, Nanotechnology, Cancer
Depositing User:	Symplectic Admin
Date Deposited:	26 Sep 2016 14:09
Last Modified:	03 Jul 2026 00:23
DOI:	10.1039/c1jm11370d
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3003452
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