Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever

Russell, George C, Scholes, Sandra F, Twomey, David F, Courtenay, Ann E, Grant, Dawn M, Lamond, Bruce, Norris, David, Willoughby, Kim, Haig, David M and Stewart, James P ORCID: 0000-0002-8928-2037 (2014) Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever. VETERINARY MICROBIOLOGY, 172 (1-2). pp. 63-71.

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Abstract

In order to define better virus isolates from animals with malignant catarrhal fever (MCF), segments of three genes of ovine herpesvirus-2 were amplified from diagnostic samples representing MCF cases with a range of clinical presentations in cattle, including head and eye, alimentary and neurological. The variation within each gene segment was estimated by DNA sequencing, which confirmed that the newly-annotated Ov9.5 gene was significantly more polymorphic than either of the other loci tested (segments of ORF50 and ORF75), with alleles that differed at over 60% of nucleotide positions. Despite this, the nine Ov9.5 alleles characterised had identical predicted splicing patterns and could be translated into Ov9.5 polypeptides with at least 49% amino acid identity. This multi-locus approach has potential for use in epidemiological studies and in charactering chains of infection. However there was no association between specific variants of OvHV-2 and the clinical/pathological presentation of MCF in the cattle analysed.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	Inflammatory disease, Malignant catarrhal fever, Sequence analysis, Multi-locus Sequence typing, Polymorphism
Depositing User:	Symplectic Admin
Date Deposited:	25 Oct 2016 11:26
Last Modified:	19 Jan 2023 07:27
DOI:	10.1016/j.vetmic.2014.04.011
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3003949