Quantification of spatiotemporal patterns of Ras isoform expression during development



Newlaczyl, AU, Coulson, JM ORCID: 0000-0003-2191-2001 and Prior, IA ORCID: 0000-0002-4055-5161
(2017) Quantification of spatiotemporal patterns of Ras isoform expression during development. Scientific reports, 7.

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Abstract

Ras proteins are important signalling hubs frequently dysregulated in cancer and in a group of developmental disorders called Rasopathies. Three Ras genes encode four proteins that differentially contribute to these phenotypes. Using quantitative real-time PCR (qRT-PCR) we have measured the gene expression profiles of each of the Ras isoforms in a panel of mouse tissues derived from a full developmental time course spanning embryogenesis through to adulthood. In most tissues and developmental stages we observe a relative contribution of KRas4B > > NRas ≥ KRas4A > HRas to total Ras expression with KRas4B typically representing 60-99% of all Ras transcripts. KRas4A is the most dynamically regulated Ras isoform with significant up-regulation of expression observed pre-term in stomach, intestine, kidney and heart. The expression patterns assist interpretation of the essential role of KRas in development and the preponderance of KRas mutations in cancer.

Item Type: Article
Uncontrolled Keywords: Cell signalling, Development, Gene expression
Depositing User: Symplectic Admin
Date Deposited: 27 Jan 2017 09:22
Last Modified: 22 Nov 2021 21:10
DOI: 10.1038/srep41297
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3005416