CLONING, SEQUENCING AND EXPRESSION OF THE OVINE INTERLEUKIN-6 GENE

EBRAHIMI, B ORCID: 0000-0002-8758-4592, ROY, DJ, BIRD, P and SARGAN, DR (1995) CLONING, SEQUENCING AND EXPRESSION OF THE OVINE INTERLEUKIN-6 GENE. CYTOKINE, 7 (3). pp. 232-236.

Text CLONING, SEQUENCING AND EXPRESSION OF OVINE INTERLEUKIN 6 GENE.pdf - Published version Download (150kB)

Abstract

Gene amplification by reverse transcriptase PCR with heterologous primers has been used to obtain a cDNA clone encoding the structural sequences of ovine interleukin 6 from alveolar macrophages. This cDNA encodes a protein of M(r) = 23,429, which is 53% homologous in amino acid sequence to human IL = 6. The clone hybridizes to an RNA of size 1260 nt in alveolar macrophages, expression of which is potentiated by LPS. The ovine IL-6 structural gene has been cloned into the yeast expression vector pOGS40, and used to produce a recombinant protein. This protein is capable of causing increased immunoglobulin production in pokeweed mitogen stimulated ovine peripheral blood mononuclear cells at concentrations of 10-100 ng/ml, but it only causes very limited replication of B9 cells, a murine IL-6 dependent cell line. This is in contrast to recombinant human IL-6, which is capable of stimulating B9 cell proliferation, but not immunoglobulin production by ovine PBMC.

Item Type:	Article
Uncontrolled Keywords:	SHEEP INTERLEUKIN 6, RECOMBINANT PROTEIN, SPECIES RESTRICTION
Depositing User:	Symplectic Admin
Date Deposited:	02 Feb 2017 11:50
Last Modified:	04 Mar 2023 00:15
DOI:	10.1006/cyto.1995.0026
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3005515