Coupling of vinculin to F-actin demands Syndecan-4 proteoglycan

Cavalheiro, RP, Lima, MA, Jarrouge-Boucas, TR, Viana, GM, Lopes, CC, Coulson-Thomas, VJ, Dreyfuss, JL, Yates, EA ORCID: 0000-0001-9365-5433, Tersariol, ILS and Nader, HB
(2017) Coupling of vinculin to F-actin demands Syndecan-4 proteoglycan. MATRIX BIOLOGY, 63. pp. 23-37.

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Syndecans are heparan sulfate proteoglycans characterized as transmembrane receptors that act cooperatively with the cell surface and extracellular matrix proteins. Syn4 knockdown was performed in order to address its role in endothelial cells (EC) behavior. Normal EC and shRNA-Syn4-EC cells were studied comparatively using complementary confocal, super-resolution and non-linear microscopic techniques. Confocal and super-resolution microscopy revealed that Syn4 knockdown alters the level and arrangement of essential proteins for focal adhesion, evidenced by the decoupling of vinculin from F-actin filaments. Furthermore, Syn4 knockdown alters the actin network leading to filopodial protrusions connected by VE-cadherin-rich junction. shRNA-Syn4-EC showed reduced adhesion and increased migration. Also, Syn4 silencing alters cell cycle as well as cell proliferation. Moreover, the ability of EC to form tube-like structures in matrigel is reduced when Syn4 is silenced. Together, the results suggest a mechanism in which Syndecan-4 acts as a central mediator that bridges fibronectin, integrin and intracellular components (actin and vinculin) and once silenced, the cytoskeleton protein network is disrupted. Ultimately, the results highlight Syn4 relevance for balanced cell behavior.

Item Type: Article
Uncontrolled Keywords: Heparan sulfate, beta 1 integrin, Fibronectin, Focal adhesion proteins, Super-resolution microscopy, Actin network
Depositing User: Symplectic Admin
Date Deposited: 06 Feb 2017 10:23
Last Modified: 19 Jan 2023 07:19
DOI: 10.1016/j.matbio.2016.12.006
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