Comparison of culture, single and multiplex real-time PCR for detection of Sabin poliovirus shedding in recently vaccinated Indian children

Giri, Sidhartha, Rajan, Anand K, Kumar, Nirmal, Dhanapal, Pavithra, Venkatesan, Jayalakshmi, Iturriza-Gomara, Miren ORCID: 0000-0001-5816-6423, Taniuchi, Mami, John, Jacob, Abraham, Asha Mary and Kang, Gagandeep (2017) Comparison of culture, single and multiplex real-time PCR for detection of Sabin poliovirus shedding in recently vaccinated Indian children. JOURNAL OF MEDICAL VIROLOGY, 89 (8). pp. 1485-1488.

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Abstract

Although, culture is considered the gold standard for poliovirus detection from stool samples, real-time PCR has emerged as a faster and more sensitive alternative. Detection of poliovirus from the stool of recently vaccinated children by culture, single and multiplex real-time PCR was compared. Of the 80 samples tested, 55 (68.75%) were positive by culture compared to 61 (76.25%) and 60 (75%) samples by the single and one step multiplex real-time PCR assays respectively. Real-time PCR (singleplex and multiplex) is more sensitive than culture for poliovirus detection in stool, although the difference was not statistically significant.

Item Type:	Article
Uncontrolled Keywords:	cell culture, poliovirus, shedding
Depositing User:	Symplectic Admin
Date Deposited:	13 Mar 2017 07:47
Last Modified:	19 Jan 2023 07:14
DOI:	10.1002/jmv.24793
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3006335