Characterization of the Prophage Repertoire of African Salmonella Typhimurium ST313 Reveals High Levels of Spontaneous Induction of Novel Phage BTP1



Owen, SV ORCID: 0000-0001-5330-3177, Wenner, N, Canals, R, Makumi, A, Hammarlof, DL, Gordon, MA ORCID: 0000-0002-0629-0884, Aertsen, A, Feasey, NA and Hinton, JCD ORCID: 0000-0003-2671-6026
(2017) Characterization of the Prophage Repertoire of African Salmonella Typhimurium ST313 Reveals High Levels of Spontaneous Induction of Novel Phage BTP1. FRONTIERS IN MICROBIOLOGY, 8.

[img] Text
Characterization of the Prophage Repertoire of African Salmonella Typhimurium ST313 Reveals High Levels of Spontaneous Induction of Novel Phage BTP1.pdf - OA Published Version

Download (6MB)
[img] Atom XML (admin)
2017-05-12T11:50:41Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T12:44:47Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T13:43:51Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T14:44:04Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T15:44:10Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T16:44:54Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T17:43:34Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T18:45:09Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T19:46:06Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T20:45:10Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T21:45:15Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T22:46:41Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-12T23:53:11Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T01:47:53Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T02:46:31Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T03:46:35Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T05:11:20Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T05:46:51Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T06:45:40Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T07:45:59Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T08:46:50Z.atom - Unspecified

Download (0B)
[img] Atom XML (admin)
2017-05-13T09:45:39Z.atom - Unspecified

Download (0B)

Abstract

In the past 30 years,Salmonella bloodstream infections have become a significant health problem in sub-Saharan Africa and are responsible for the deaths of anestimated 390,000 people each year. The disease is predominantly caused by a recently described sequence type of SalmonellaTyphimurium: ST313, which has a distinctive set of prophage sequences. We have thoroughly characterized the ST313-associated prophages both genetically and experimentally. ST313 representative strain D23580 contains five full-length prophages: BTP1, Gifsy-2D23580, ST64BD23580, Gifsy-1D23580,and BTP5. We show that commonS.Typhimurium prophages Gifsy-2, Gifsy-1, andST64B are inactivated in ST313 by mutations. Prophage BTP1 was found to be a functional novel phage, and the first isolate of the proposed new species “Salmonellavirus BTP1”, belonging to the P22virusgenus. Surprisingly,∼109BTP1 virus particlesperml were detected in the supernatant of non-induced, stationary-phase culturesof strain D23580, representing the highest spontaneously induced phage titer so farreported for a bacterial prophage. High spontaneous induction is shown to be anintrinsic property of prophage BTP1, and indicates the phage-mediated lysis of around0.2% of the lysogenic population. The fact that BTP1 is highly conserved in ST313 poses interesting questions about the potential fitness costs and benefits of novel prophagesin epidemicS.Typhimurium ST313.

Item Type: Article
Uncontrolled Keywords: Gifsy, ST64B, BTP5, P22, polylysogeny, D23580
Depositing User: Symplectic Admin
Date Deposited: 24 Mar 2017 10:24
Last Modified: 14 Oct 2021 12:09
DOI: 10.3389/fmicb.2017.00235
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3006596