Human Fetal Osteoblast Response on Poly(Methyl Methacrylate)/Polystyrene Demixed Thin Film Blends: Surface Chemistry Vs Topography Effects

D'Sa, RA ORCID: 0000-0003-2651-8783, Raj, J, Dickinson, PJ, McCabe, F and Meenan, BJ (2016) Human Fetal Osteoblast Response on Poly(Methyl Methacrylate)/Polystyrene Demixed Thin Film Blends: Surface Chemistry Vs Topography Effects. ACS APPLIED MATERIALS & INTERFACES, 8 (24). pp. 14920-14931.

Text
osteoblast paper final.pdf - Author Accepted Manuscript
Download (1MB)

Official URL: https://pubs.acs.org/doi/10.1021/acsami.5b08073

Abstract

Recent advances in materials sciences have allowed for the development and fabrication of biomaterials that are capable of providing requisite cues to instigate cells to respond in a predictable fashion. We have developed a series of poly(methyl methacrylate)/polystyrene (PMMA/PS) polymer demixed thin films with nanotopographies ranging from nanoislands to nanopits to study the response of human fetal osteoblast cells (hFOBs). When PMMA was in excess in the blend composition, a nanoisland topography dominated, whereas a nanopit topography dominated when PS was in excess. PMMA was found to segregate to the top of the nanoisland morphology with PS preferring the substrate interface. To further ascertain the effects of surface chemistry vs topography, we plasma treated the polymer demixed films using an atmospheric pressure dielectric barrier discharge reactor to alter the surface chemistry. Our results have shown that hFOBs did not have an increased short-term cellular response on pristine polymer demixed surfaces. However, increasing the hydrophilicty/wettability of the surfaces by oxygen functionalization causes an increase in the cellular response. These results indicate that topography alone is not sufficient to induce a positive cellular response, but the underlying surface chemistry is also important in regulating cell function.

Item Type:	Article
Uncontrolled Keywords:	surface topography, surface chemistry, polymer demixing, cellular response, human fetal osteoblasts, plasma surface modification
Depositing User:	Symplectic Admin
Date Deposited:	31 Mar 2017 13:06
Last Modified:	19 Jan 2023 07:07
DOI:	10.1021/acsami.5b08073
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3006734