Characterising molecular mechanisms of Crohn's disease-associated Escherichia coli that enable their survival and replication within macrophages

Tawfik, Ahmed (2016) Characterising molecular mechanisms of Crohn's disease-associated Escherichia coli that enable their survival and replication within macrophages PhD thesis, University of Liverpool.

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Abstract

Mucosa-associated adherent, invasive Escherichia coli (AIEC), found in increased number in Crohn’s disease (CD) ileal and colonic mucosae, can survive and replicate within underlying host immune competent cells (e.g. macrophages and dendritic cells) without triggering host cell death. The intra-macrophage environment plays an essential role in bacterial killing where engulfed bacteria are exposed to a hostile environment of low pH, high levels of proteolytic/lysosomal enzymes, high nitrosative and high oxidative stress, and the activation of a respiratory burst with generation of superoxide ions. Although a few stress response genes have been identified that likely support the paradigm ileal AIEC isolate LF82 to survive and replicate within the macrophage, the key molecular mechanisms involved in supporting Crohn’s disease (CD) mucosa-associated AIEC to resist killing by host mucosal macrophages within harsh environment of the phagolysosome still remains largely unclear. Here we aimed to compare the ability of a number of E. coli strains to survive and replicate inside macrophages, including a number of clinical isolates (from CD, colorectal cancer (CRC) and ulcerative colitis (UC) patients and other infective ... (continues)

Item Type:	Thesis (PhD)
Divisions:	Faculty of Health & Life Sciences
Depositing User:	Symplectic Admin
Date Deposited:	23 Aug 2017 09:42
Last Modified:	05 Aug 2025 08:46
DOI:	10.17638/03007136
Supervisors:	Campbell, BJ ORCID: 0000-0002-7407-012X Rhodes, JM ORCID: 0000-0002-1302-260X
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3007136
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