Assessing the relationship between nanoparticle physicochemical characteristics and biological interactions : optimisation of in vitro techniques and protocols

David, Christopher AW ORCID: 0000-0001-8504-2354 (2016) Assessing the relationship between nanoparticle physicochemical characteristics and biological interactions : optimisation of in vitro techniques and protocols. PhD thesis, University of Liverpool.

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Abstract

The development and implementation of nanomaterials for a variety of clinical applications is increasing as their utility in improving healthcare is demonstrated. However, consideration must be given to appropriate pre-clinical testing to fully translate these materials into clinical use. A library of 22 nanomaterials, both commercially available and those developed in-house, were subject to an assay cascade forming the basis of a preclinical in vitro assessment which utilised a broad and widely accessible range of techniques. The library comprised numerous material classes; metallic (gold, silver, iron oxide, titanium dioxide, zinc oxide), non-metal (silica), and polymeric (polystyrene, liposome, emulsion, polydendron), varying in manufacturer stated particle size, charge, and functionalization. Chapter 2 details characterisation of the size and zeta potential of the nanomaterial library in biologically relevant matrices. When combined with information provided by the manufacturers regarding stabilisation and surface functionalization, where available, these measures allowed associations to be made between nanoparticle physicochemical characteristics and the biological effects observed in subsequent chapters. ... (continues)

Item Type:	Thesis (PhD)
Divisions:	Faculty of Health and Life Sciences > Faculty of Health and Life Sciences
Depositing User:	Symplectic Admin
Date Deposited:	23 Aug 2017 09:19
Last Modified:	02 Nov 2024 14:33
DOI:	10.17638/03007382
Supervisors:	Liptrott, N ORCID: 0000-0002-5980-8966 Owen, A ORCID: 0000-0002-9819-7651 Rannard, S ORCID: 0000-0002-6946-1097
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3007382