Calcium phosphate substrates with emulsion-derived roughness: Processing, characterisation and interaction with human mesenchymal stem cells

Costa Machado, G, García-Tuñón, E, Bell, RV, Alini, M, Saiz, E and Peroglio, M (2017) Calcium phosphate substrates with emulsion-derived roughness: Processing, characterisation and interaction with human mesenchymal stem cells. Journal of the European Ceramic Society, 38 (3). pp. 949-961.

Text processing_cells_rev.docx - Author Accepted Manuscript Download (170kB)

Abstract

Calcium phosphates (CaP) have been the subject of several studies that often lack a systematic approach to understanding how their properties affect biological response. CaP particles functionalised with a pH-responsive polymer (BCS) were used to prepare microporous substrates (porosity between 70 and 75% and pore sizes of 5–20 μm) through the aggregation of oil-in-water emulsions by controlling solid loading, emulsification energy, pH, drying and sintering conditions. The combined effect of surface roughness (roughness amplitude, Ra between 0.9–1.7 μm) and chemistry (varying Hydroxyapatite/β-Tricalcium phosphate ratio) on human mesenchymal stem cells was evaluated. HA substrates stimulated higher cell adhesion and proliferation (especially with lower Ra), but cell area increased with β-TCP content. The effect of surface roughness depended of chemistry: HA promoted higher mineralising activity when Ra ∼ 1.5 μm, whereas β-TCP substrates stimulated a more osteogenic profile when Ra ∼ 1.7 μm. A novel templating method to fabricate microporous CaP substrates was developed, opening possibilities for bone substitutes with controlled features.

Item Type:	Article
Uncontrolled Keywords:	Calcium phosphates, Emulsions, Microporosity, Surface roughness, Human mesenchymal stem cells
Depositing User:	Symplectic Admin
Date Deposited:	04 Jul 2017 09:50
Last Modified:	19 Jan 2023 07:00
DOI:	10.1016/j.jeurceramsoc.2017.06.043
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3008319