Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> ST410 in the United Kingdom



Timofte, Dorina ORCID: 0000-0002-7261-738X, Maciuca, Iuliana Elena, Williams, Nicola J, Wattret, Andrew and Schmidt, Vanessa ORCID: 0000-0001-5460-6217
(2016) Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> ST410 in the United Kingdom. MICROBIAL DRUG RESISTANCE, 22 (7). pp. 609-615.

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Abstract

We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified bla<sub>CTX-M</sub> to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried bla<sub>CTX-M-15</sub> and bla<sub>CTX-M-14,</sub> respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of bla<sub>CTX-M-15</sub>. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried bla<sub>CTX-M-15</sub>, and co-harbored bla<sub>OXA-1,</sub> bla<sub>TEM-1,</sub> bla<sub>CMY-2,</sub> and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15-producing E. coli ST410.

Item Type: Article
Uncontrolled Keywords: E, coli, ESBL, surveillance, veterinary, infection control
Depositing User: Symplectic Admin
Date Deposited: 25 Sep 2017 15:07
Last Modified: 12 Oct 2023 10:57
DOI: 10.1089/mdr.2016.0036
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3008355