Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom

Timofte, Dorina ORCID: 0000-0002-7261-738X, Maciuca, Iuliana Elena, Williams, Nicola J, Wattret, Andrew and Schmidt, Vanessa ORCID: 0000-0001-5460-6217 (2016) Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom MICROBIAL DRUG RESISTANCE, 22 (7). pp. 609-615. ISSN 1076-6294, 1931-8448

Text Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom.pdf - Published version Download (310kB)

Abstract

We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified bla<inf>CTX-M</inf> to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried bla<inf>CTX-M-15</inf> and bla<inf>CTX-M-14,</inf> respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of bla<inf>CTX-M-15</inf>. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried bla<inf>CTX-M-15</inf>, and co-harbored bla<inf>OXA-1,</inf> bla<inf>TEM-1,</inf> bla<inf>CMY-2,</inf> and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15-producing E. coli ST410.

Item Type:	Article
Uncontrolled Keywords:	E, coli, ESBL, surveillance, veterinary, infection control
Depositing User:	Symplectic Admin
Date Deposited:	25 Sep 2017 15:07
Last Modified:	23 May 2026 00:07
DOI:	10.1089/mdr.2016.0036
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3008355
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