Insights into the role of 3-O-sulfotransferase in heparan sulfate biosynthesis

Zorel Meneghetti, MC, Ferreira, TG, Tashima, AK, Chavante, SF, Yates, EA ORCID: 0000-0001-9365-5433, Liu, J, Nader, HB and Lima, MA
(2017) Insights into the role of 3-O-sulfotransferase in heparan sulfate biosynthesis. ORGANIC & BIOMOLECULAR CHEMISTRY, 15 (32). 6792 - 6799.

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3-O-Sulfotransferase enzyme (sHS) from Litopenaeus vannamei was cloned and its substrate specificity was investigated against a number of GAG structures, including modified heparin polysaccharides and model oligosaccharides. For the heparin polysaccharides, derived from porcine intestinal mucosa heparin, sulfate groups were incorporated into glucosamine residues containing both N-sulfated and N-acetylated substitution within the regions of the predominant repeating disaccharide, either I–ANS or I–ANAc. However, the resulting polysaccharides did not stabilize antithrombin, which is correlated with anticoagulant activity. It was also shown that the enzyme was able to sulfate disaccharides, I2S–ANS and G–ANAc. The results further illustrate that 3-O-sulfation can be induced outside of the classical heparin-binding pentasaccharide sequence, show that 3-O-sulfation of glucosamine is not a sufficient condition for antithrombin stabilization and suggest that the use of this enzyme during HS biosynthesis may not occur as the final enzymatic step.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 11 Aug 2017 09:40
Last Modified: 07 Sep 2022 03:10
DOI: 10.1039/c7ob01533j
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