Role of activation-induced cytidine deaminase (AID) in follicular lymphoma biology

Alishlash, OA (2017) Role of activation-induced cytidine deaminase (AID) in follicular lymphoma biology. PhD thesis, University of Liverpool.

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Abstract

Follicular lymphoma is the second most common non-Hodgkin’s lymphoma (NHL). The clinical course of disease is heterogeneous, typically with multiple relapses. Most patients live 10 years or more. However, another group of patients deteriorate rapidly and may progress to death within two years. Activation induced cytidine deaminase (AID) is an enzyme that plays an important role in somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes (IG). It induces mutations in IG and non-IG genes leading to genomic instability and chromosomal breaks that are important in the pathogenesis of B-cell malignancies. In this study, we wanted to first measure AID mRNA and protein levels and its biological function in follicular lymphoma (FL) and then correlate each of these variables with clinical features. Our cohort consisted of 87 patients recruited into the Purine-Alkylator Combination In Follicular lymphoma Immuno-Chemotherapy for Older patients (PACIFICO) trial which is comparing alternative frontline chemoimmunotherapy regimens in older patients with FL. The patient samples were in the form of formalin fixed, paraffin embedded (FFPE) biopsies, which are notorious for nucleic acid degradation. ... (continues)

Item Type:	Thesis (PhD)
Divisions:	Faculty of Health and Life Sciences > Faculty of Health and Life Sciences
Depositing User:	Symplectic Admin
Date Deposited:	14 Dec 2017 12:09
Last Modified:	30 Sep 2024 02:15
DOI:	10.17638/03009367
Supervisors:	Pettitt, A ORCID: 0000-0002-0907-8950 Coupland, SE ORCID: 0000-0002-1464-2069 Lin, KE
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3009367