Remote regulation of magnetic particle targeted Wnt signaling for bone tissue engineering

Rotherham, Michael, Henstock, James R, Qutachi, Omar and El Haj, Alicia J
(2018) Remote regulation of magnetic particle targeted Wnt signaling for bone tissue engineering. Nanomedicine: Nanotechnology, Biology and Medicine, 14 (1). pp. 173-184.

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Wnt signaling is critically involved in the differentiation of human Mesenchymal Stem Cells (hMSC). Wnt proteins therefore have considerable therapeutic value, but are expensive and difficult to produce. UM206 is a synthetic peptide and ligand for the Wnt receptor Frizzled. Attachment of UM206 to magnetic nanoparticles (MNP) enables the ligand-MNP complex to be manipulated using magnetic fields, allowing control of Frizzled stimulation. Using this approach, Wnt signaling was activated in hMSC which resulted in Frizzled clustering, β-catenin translocalization and activation of TCF/LEF responsive transcription. During osteogenesis, UM206-MNP initiated localized mineralized matrix formation. Injection and magnetic stimulation of UM206-MNP-labeled MSC in ex vivo chick femurs resulted in increased mineralization which acted synergistically with addition of bone morphogenic protein 2 (BMP2) releasing micro-particles. As this facilitates external control over signal transduction, conjugated MNP technology has applications both as a research tool and for regulating tissue formation in clinical cell therapies.

Item Type: Article
Uncontrolled Keywords: Mesenchymal stem cells, Magnetic nanoparticles, Wnt signaling, Bone tissue engineering
Depositing User: Symplectic Admin
Date Deposited: 10 Oct 2017 07:17
Last Modified: 19 Jan 2023 06:53
DOI: 10.1016/j.nano.2017.09.008
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