Identification of Equid herpesvirus 2 in tissue-engineered equine tendon

Wardle, R, Pullman, JA, Haldenby, S, Ressel, L, Pope, M, Clegg, PD, Radford, A, Stewart, JP, Al-Saadi, M, Dyer, P
et al (show 1 more authors) (2017) Identification of Equid herpesvirus 2 in tissue-engineered equine tendon. Wellcome Open Research, 2. 60 - 60. ISSN 2398-502X

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Additional Information: referee-status: Approved with reservations, Approved with reservations referee-response-24795: 10.21956/wellcomeopenres.13180.r24795, Duncan Hannant, School of Veterinary Medicine and Science, University of Nottingham, Loughborough, UK, 29 Aug 2017, version 1, 2 approved with reservations referee-comment-3109: <b>Mandy Peffers</b>; <i>Posted: 13 Oct 2017</i>; The authors appreciate the comments made by the reviewer. We have taken on board their concerns and amended the manuscript accordingly to include discussion of other possible reasons for the findings of EHV-2 in equine tenocytes. referee-response-26237: 10.21956/wellcomeopenres.13180.r26237, Sarah Elizabeth Taylor, The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, UK, 09 Oct 2017, version 1, 2 approved with reservations referee-comment-3108: <b>Mandy Peffers</b>; <i>Posted: 13 Oct 2017</i>; ReviewerThis study suggests that a primary viral infection coupled with subsequent bacterial infection leads to catastrophic musculoskeletal infection and death&quot; Please clarify that this is&nbsp;the study on broiler hens and not the current work.ResponseWe have amended the manuscript to clarify that this was indeed in broiler hens.ReviewerIt is not clear from the current work what statistical tests were carried out in the programmes described in the materials and methods.ResponseWe have amended the manuscript to clarify that these statistical tests relate to the hydroxyproline assay.ReviewerWas there any RNA from the virus infected tenocytes left over for analysis?ResponseAll the RNA samples were used in the studies pertaining to this manuscript.ReviewerDid the authors collect these tenocytes from tissue that was known to be septic antemortem? I wonder if this statement is a jump too far.ResponseAll samples were grossly normal on collection. Hospital derived samples showed no signs of sepsis.ReviewerWere any of the RNA seq hits for the latency associated transcript RNA?ResponseTwo different types of mapping were undertaken. Firstly a megablast run against the entire nt database to get taxonomic information, and &nbsp;secondly a blastx run against a database &nbsp;created of all know ERV genes in animals from NCBI. Therefore the’ latency associated transcripts’ (only appear to be sequences for these in humans in NCBI) would not have been included. We assessed the two sequences in NCBI that result from a search for 'latency associated transcript and herpes' and ran a blastx for both samples against these. Neither resulted in any significant hits. &nbsp;Thus whilst latency associated transcripts cannot be ruled out with data currently available this is unlikely. A more in-depth analysis than this would require further research in latency associated transcripts linked to herpes in other animals and is beyond the scope of this paper.&nbsp; We have amended the manuscript accordingly.ReviewerThe specific PCR for EHV-2 on tendon tissue did not find any viral DNA however can this test confirm lack of presence of&nbsp;latency associated transcript RNA?ResponseThe reviewer is correct in their assumption. We have amended the manuscript. grant-information: This work was supported by the Wellcome Trust [107471]; the Medical Research Council (MRC) and Arthritis Research UK as part of the MRC-Arthritis Research UK Centre for Integrated research into Musculoskeletal Ageing (CIMA), and by a Wellcome Trust Summer Studentship. copyright-info: This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Depositing User: Symplectic Admin
Date Deposited: 20 Oct 2017 09:50
Last Modified: 02 Feb 2018 12:11
DOI: 10.12688/wellcomeopenres.12176.1
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