Bower, Danielle V, Lansdale, Nick, Navarro, Sonia, Truong, Thai V, Bower, Dan J, Featherstone, Neil C, Connell, Marilyn G, Al Alam, Denise, Frey, Mark R, Trinh, Le A et al (show 5 more authors)
(2017)
SERCA directs cell migration and branching across species and germ layers.
BIOLOGY OPEN, 6 (10).
pp. 1458-1471.
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Abstract
Branching morphogenesis underlies organogenesis in vertebrates and invertebrates, yet is incompletely understood. Here, we show that the sarco-endoplasmic reticulum Ca<sup>2+</sup> reuptake pump (SERCA) directs budding across germ layers and species. Clonal knockdown demonstrated a cell-autonomous role for SERCA in <i>Drosophila</i> air sac budding. Live imaging of <i>Drosophila</i> tracheogenesis revealed elevated Ca<sup>2+</sup> levels in migratory tip cells as they form branches. SERCA blockade abolished this Ca<sup>2+</sup> differential, aborting both cell migration and new branching. Activating protein kinase C (PKC) rescued Ca<sup>2+</sup> in tip cells and restored cell migration and branching. Likewise, inhibiting SERCA abolished mammalian epithelial budding, PKC activation rescued budding, while morphogens did not. Mesoderm (zebrafish angiogenesis) and ectoderm (<i>Drosophila</i> nervous system) behaved similarly, suggesting a conserved requirement for cell-autonomous Ca<sup>2+</sup> signaling, established by SERCA, in iterative budding.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Branching morphogenesis, Cell migration, SERCA, Calcium dynamics |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 15 Nov 2017 15:20 |
| Last Modified: | 19 Jan 2023 06:50 |
| DOI: | 10.1242/bio.026039 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3012231 |
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