Live Imaging of Cell Invasion Using a Multicellular Spheroid Model and Light-Sheet Microscopy.

Marcello, M ORCID: 0000-0002-2392-8640, Richards, R, Mason, D ORCID: 0000-0002-8773-5274 and Sée, V (2017) Live Imaging of Cell Invasion Using a Multicellular Spheroid Model and Light-Sheet Microscopy. Advances in experimental medicine and biology, 1035. pp. 155-161.

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Abstract

Three-dimensional cellular assays are becoming increasingly popular as a fundamental tool to bridge the gap between tissue culture systems and in vivo tissue. In particular, spheroids are recognised today as a necessary intermediate model between testing in monolayer cultures and testing in animals. This chapter describes a straightforward protocol, from sample preparation to image acquisition and initial post-processing, based on one of most widely used commercial light-sheet fluorescence microscopy platform, the Zeiss Lightsheet Z.1.

Item Type:	Article
Uncontrolled Keywords:	Multicellular spheroid, Light-sheet fluorescence microscopy, Cell invasion, FEP tubes, Matrigel, Hanging drop, Glioblastoma, Multiview reconstruction
Depositing User:	Symplectic Admin
Date Deposited:	29 Nov 2017 08:16
Last Modified:	19 Jan 2023 06:49
DOI:	10.1007/978-3-319-67358-5_11
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3013086