Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue‐engineered skin equivalents



Smith, Sarah, Colley, Helen, Sharma, P ORCID: 0000-0002-5534-2417, Slowik, Klaudia, Sison-Young, Rowena, Sneddon, Andrew, Webb, Steven and Murdoch, Craig
(2018) Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue‐engineered skin equivalents. Experimental Dermatology, 27 (5). 473 - 475.

This is the latest version of this item.

[img] Text
Smith_et_al-2017-Experimental_Dermatology.pdf - Accepted Version

Download (759kB)
[img] Text
Smith_et_al-2018-Experimental_Dermatology.pdf - OA Published Version

Download (351kB)

Abstract

CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue‐engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl‐O‐methyl‐cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time‐dependent increase in metabolite production, confirming the functional expression of these enzymes in skin.

Item Type: Article
Uncontrolled Keywords: epithelium, steroids, three-dimensional tissue models, toxicity, xenobiotic metabolism
Depositing User: Symplectic Admin
Date Deposited: 14 Dec 2017 09:34
Last Modified: 19 Jan 2022 08:19
DOI: 10.1111/exd.13483
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3014132

Available Versions of this Item