Immunohistochemical Expression of Heat Shock Proteins in Fibroblastic/ Myofibroblastic Tumours of Extremities

Al Amri, A
(2017) Immunohistochemical Expression of Heat Shock Proteins in Fibroblastic/ Myofibroblastic Tumours of Extremities. Master of Philosophy thesis, University of Liverpool.

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BACKGROUND: Soft tissue sarcomas (STS) are rare heterogeneous human malignant neoplasms, accounting for 1% of all human malignancies, arising mostly from various mesenchymal tissue. Heat shock proteins (HSPs) are involved in cell proteostasis, and their expression by cancer cells allows the cells to survive, invade and undergo metastasis. AIMS: The purpose of this thesis was to investigate the potential role of key HSPs (HSP27, HSP70 and HSP90) in fibroblastic/myofibroblastic tumours of the extremities. METHODS: Expression of HSP27, HSP70 and HSP90 were analysed using immunohistochemistry on tissue sections from 35 cases of fibroblastic/myofibroblastic sarcomas and 8 cases of non-metastasising fibroblastic tumours. The expression of each protein was evaluated and its relationship with clinicopathological parameters examined. RESULTS: HSP27 and HSP70 were expressed in all fibroblastic/myofibroblastic tumours, but only the expression of HSP70 was significantly higher in sarcomas compared to non-metastasising counterparts (P < 0.05). Also, there was a significant relationship between HSP70 expression and the grade of sarcomas (P < 0.05). However, no significant association found between HSP27 and HSP90 expression and clinicopathological features of sarcomas. CONCLUSIONS: The findings of this study indicate that HSP70, and to a lesser extent HSP27, might have a role in sarcoma behaviour (as defined by the surrogates of tumour type and grade) and may provide prognostic information for clinicians in the future. Further studies are warranted to gain more insight into the role of these HSPs in the tumorigenesis of soft tissue sarcomas to determine whether they are possible markers of prognosis.

Item Type: Thesis (Master of Philosophy)
Depositing User: Symplectic Admin
Date Deposited: 16 Aug 2018 11:22
Last Modified: 17 Jan 2024 17:07
DOI: 10.17638/03018038